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    浮游植物分类荧光仪——PHYTO-PAM-II
    日期:2017-01-04 22:12:05

    主要功能

    ●对自然水体中的蓝藻、绿藻、硅/甲藻和隐藻自动分类(定性)

    ●自动测量水样中蓝藻、绿藻、硅/甲藻和隐藻的叶绿素 a 含量(定量)和总叶绿素 a 含量

    ●一杯自然水样,同时获得蓝藻、绿藻、硅/甲藻和隐藻的光合活性:

    ●光合效率和光合速率(相对电子传递速率)

    ●快速光曲线并进行拟合

    ●藻类的潜在最大光合效率

    ●藻类的光保护能力

    ●藻类耐受强光的能力

    ●用户可做自己的参考光谱


    测量参数

    Fo, Fm, F, Fm', Fv/Fm, Y(II) 即 ΔF/Fm', ETR, a, Ik, Pm, PAR 和叶绿素含量等

     

    应用领域

    ●主要用于水生生物学、水域生态学、海洋学、湖沼学、水质预警、微藻生理学、微藻抗逆性等领域,对于了解自然水样中藻类种群的动态变化、水华预警、野外水体中光合作用的时空变化、校正初级生产力的计算等有较大帮助。

    ●特别适于浮游植物动力学研究和有害水华的早期预警。

     

    主要技术参数

    ●主控单元:金属外壳,包含所有的光电元件及样品测量室

    ●测量光: LED,440 nm,480 nm,540 nm,590 nm 和 625 nm,5 波长脉冲调制测量光,2 档强度设置,8 档调制频率设置, 3 档测量光自动高频设置

    ●光化光:板载多波长 LED 阵列,440 nm,480 nm,540 nm,590 nm,625 nm 和 420-640 nm(白光),提供持续光化光,最高可达 1400 μmol m-2 s-1,快速动力学闪光高达 7000 μmol m-2 s-1,饱和脉冲最高可达 5000 μmol m-2 s-1

    ●远红光:725 nm LED

    ●信号检测:基于 H-10720 光电传感器模块的光电倍增检测器

    ●标准检测过滤器:> 650 nm 长通滤光片

    ●高分辨率的光电倍增管,叶绿素浓度检测限低至 0.1μg/L,适用野外采集的藻浓度很稀的样品

     

    升级的技术特点

    ●可提供 5 种波长的脉冲调制测量光和光化光

    ●板载 LED 阵列芯片技术

    ●可实时进行四种藻的分类

    ●可进行标准 PAM 测量及不同波长强光化光诱导的毫秒级荧光上升动力学分析

    ●可测定光系统 II 功能性捕光截面积

    ●内置自动测量程序,易于操作

     

    PHYTO-PAM-II vs PHYTO-PAM

      便携式 PHYTO-PAM-II

      PHYTO-PAM

      5 种不同波长的测量光用于生物体内不同类型的天线色素荧光的激发

      4 种不同波长的光

      4 种色素类型的在线分类

      3 种色素类型的在线分类

      6 种波长的光化光

      1 种波长的光化光

      可以分别测量绿藻,蓝藻,硅/甲藻以及含有藻红蛋白的有机体,如隐藻不同波长下 PSII 的活性

      无该功能

      紧凑型设计

      需要组装

      增加了快速动力学操作模式,可通过强光化光脉冲,测量不同波长的 O- I1 荧光上升动力学曲线

      无该功能

      通过测定不同光质和光合生物色素复合体,获得光系统 II 功能性捕光截面积的信息即 σPSII

      无该功能

      通过 FluoRed 荧光标准将参考光谱校准标准化

      无该功能

      获得的参考光谱可在不同设备及用户间互换使用

      参考光谱不能互换

      

    选购指南

    一、实验室测量基本款

    系统组成:实验室版主机,激发检测单元,悬浮液的光学单元,球形光量子传感器,工作台,软件等

    注意:新版 PHYTO-PAM-II 必须配置光量子传感器,校准光强后参考光谱可在不同仪器间通用

    Phyto-PAM II laboratory version.jpg

    实验室测量基本款

      

    二、野外便携紧凑款

    系统组成:紧凑型主机,球形光量子传感器,软件等

    注意:新版 PHYTO-PAM-II 必须配置光量子传感器,校准光强后参考光谱可在不同仪器间通用

    Phytho-PAMM-II_071_1-small-2.jpg

    便携式紧凑款


    Channels.jpg

    algae.jpg

    Reference.jpg

    五种测量光通道

    区分四种藻类

    通用型参考光谱

    软件界面7.png

    light-curve.jpg

    Fast-Kinetics.jpg

    测量慢速动力学曲线

    淬灭分析,暗弛豫分析

    测量快速光曲线

    光响应曲线

    测量快速动力学曲线

    分析PSII功能性捕光截面


    三、其他可选附件

    1,WATER-S:搅拌器(推荐选配),利用内置电池驱动的马达带动搅拌棒旋转,对样品杯里的悬浮液进行搅拌。需配置搅拌棒 WATER-R。

    2,WATER-R:搅拌棒(标配10根)

     

    产地:德国WALZ


    参考文献

    数据来源:光合作用文献 Endnote 数据库,更新至 2021 年 1 月,文献数量超过 10000 篇

    原始数据来源:Google Scholar

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